RDW blood test
Red blood cell (RBC) indices are calculations derived from the complete blood count that helps in the process of diagnosis and classification of Anemia. A RBC indices blood test requires 3 to 5 ml of blood collected from a vein puncture.
RDW (Red cell Distribution Width) blood test that calculates any variation in size of RBC. Normally Red Blood Cells are of a standard size. Since RBCs tend to get smaller and rounder as they age, it is not possible for all cells to be exactly the same size. RDW test determines the changes in the volume of the cells in the red cell population. The variations pertaining to the size of the red blood cells is called Anisocytosis and the shape is called Poikilocytosis.
The incidence of these conditions and the detection of abnormal or varied red blood size are determined by diagnostic blood smear analysis and also through automated analyzers.
The following are the normal results for red blood cell indices ( a measurement of the variation in size of red blood cells):
Normochromic: Indicates a normal concentration of hemoglobin. The RDW increases in anemia caused by deficiencies of Vitamin B12, iron or folic acid. Abnormal hemoglobin, as in sickle cell anemia, can result in changes in the shape of red blood cells. In addition it may also cause them to hemolyze. The abnormal shape and the cell fragments as a result of hemolysis would tend to increase the RDW. Severe blood loss may also induce immature cells into the blood that can increase the RDW.
Possible causes of abnormal MCV: MCV (Mean Corpuscular Volume) is an index of the size of the RBC. When the MCV is below normal, the RBC will be smaller than the normal size - which is known as microcytic. On the other hand, if the MCV is higher than normal, the RBC will also be larger than normal which is called as macrocytic while the normal RBC size is termed as normocytic.
High MCV: Macrocytic anemia owing to vitamin B12 and folic acid deficiency
Low MCV: Microcytic anemia, Thalassemia, poor iron content in diet and chronic illness
Normal MCV: Normocytic anemia owing to liver,kidney diseases, bone marrow disorders, hemolysis (distruction of red blood cells) or excessive bleeding
A low MCH (Mean Corpuscular Hemoglobin) is an indication that RBCs have too little hemoglobin. When viewed under the microscope, the RBCs appear pale and hence called as hypochromic. The MCHC (Mean Corpuscular Hemoglobin Concentration) is the ratio of RBC's hemoglobin mass to the cell volume. MCHC is elevated in hereditary Spherocytosis - as a result of the structural protein defect in the RBC.
Calculating Red Blood Cell Distribution: The determination of red cell distribution width is mathematically expressed as Red cell distribution width = (Standard deviation ÷ Mean cell volume) X 100.
Conditions such as pernicious anemia cause changes in RBC size. Often there is variation in shape too. This can be detected by changes in RDW. Normal RDW range is 11 - 15%. Higher RDW is indicative of disorders such as anemia. RDW blood test is often conducted along with MCV blood test to check the exact cause.
Hemolytic anemia is a kind of anemia that occurs when there is high rate of destruction of RBC. Infections of Streptococcus or other conditions can lead the body to destroy its own RBC. Hemolytic anemia can enlarge the spleen that results in increased destruction of red blood cells (Hemolysis). Some complications of this Anemia:
Persons suffering hemolytic condition might experience severe fatigue, dizziness, shortness of breath and chest pain. A complete blood count can help in identifying hemolytic anemia. Hemolytic anemia is treated with lifestyle changes and medicines. In more severe cases blood transfusion and plasmapheresis might be resorted to.
Autoimmune Hemolytic Anemia : Warm antibody hemolytic anemia, one of the most common types afflicting women, results when the body produces auto antibodies that coat red blood cells which in turn are destroyed by the spleen, liver or bone marrow. Many of these patients also have leukemia, lupus, lymphoma or connective tissue disease. In cold antibody hemolytic anemia, the body targets red blood cells at or below normal body temperature - often to patients who have had pneumonia, mononucleosis or other acute infections. Exposure to cold temperatures can accelerate red blood cell destruction resulting in fatigue, discoloration of limbs and joint pains.
Sideroblastic Anemia: In this anemia, the body has sufficient iron but it fails to incorporate it into hemoglobin.
MCV blood test
MCV (Mean Corpuscular Volume) blood test measures the size of RBC. It is a measure of the average volume of a red blood cell by dividing the hematocrit by the RBC. Any change in the size of RBC indicates certain disorders. This test is often conducted along with RDW blood test. With MCV blood test, RBCs can be categorized into normal, small or large sizes. Larger cells are usually indicative of macrocytic anemia. On the other hand, those with small cells may suffer microcytic anemia. Larger RBCs may indicate liver disease, hypothyroidism, folic acid or vitamin B12 deficiency or marrow aplasia. Decreased size of red blood cells is usually noticed in persons suffering from anemia, thalassemia, lead poisoning or chronic renal failure.
MCV Blood test range
MCV blood test is done using electrical impedance or light deflection. The MCV values are considered as significant and reliable in the clinical correlation of a disease. The standard reference value for MCV is 80-100 femolitres. The reference range varies from person to person depending on the age group. In children the reference range for MCV is given in three categories:
For Newborns (95 to 121 fl)
For Infants and Toddlers (76- 86 fl)
For Teenagers (boys: 78- 98 fl and girls 78-102fl)
For Adults 98fl
The MCV in association with underlying anemic conditions is determined by the following mathematical formulae: MCV = 10 X (packed cell volume / RBC count)
Mentzer index is mathematically expressed as MCV / RBC count. The Mentzer index value determines conditions such as thalassemia when the ratio is less than 13 and it indicates iron deficiency anemia when the ratio is greater than 13.
Bibliography / Reference
Collection of Pages - Last revised Date: December 18, 2018