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Urine Amino Acid Analysis

Amino acids play a vital role as building blocks of proteins and also as intermediates in metabolism. These amino acids help in neurotransmitter function, pH balance regulation, hormone metabolism, pain and inflammation control, detoxification, cholesterol metabolism and various other biological activities. Excess or deficiency of amino acids in the body either due to dietary restriction or inherited metabolic problems can cause very serious health issues.


Urine amino acid test measures the amino acid levels in the urine. It is a medical test that helps in identifying underlying chronic disorders caused by amino acid imbalances and vitamin and mineral deficiencies. Most often, Amino acid test is performed as a screening test in newborn and children to address metabolism disorders. If any of the essential amino acids are not available in sufficient quantities, the production of protein is compromised with inefficient metabolism. The deficiency may cause disorders such as ADD, depression, Tourette syndrome, tic disorder, OCD, seizures and others.


Urine amino acid test provides valuable information on amino acid imbalance in the body by identifying:

Dietary protein adequacy
Gastrointestinal dysfunction
Enzyme functionality
Protein intolerance
Vitamin and mineral deficiencies
Renal and hepatic dysfunction, psychiatric abnormalities
Susceptibility to degenerative disorders
Inflammatory response and oxidative stress
Reduced detoxification capacity and many other inherent and acquired disorders

There are around 20 different amino acids in the urine. If the urine indicates any one or two of the amino acid levels as higher than normal, the person may have an inherent error of metabolism. Diagnosing it during infancy can prevent problems such as brain damage.


No prior preparation is required for the test. Clean catch urine sample is collected to perform the test. Few laboratories ask for first morning urine and few other prefer 24-hour urine test for assessing the amino acid levels. 24 hour urine test gives a detailed picture of highs and lows of various amino acid levels through the day, whereas first morning urine identifies current amino acid status and any existing imbalances. Reports are presented in a tabular format grouped into functional categories clearly indicating:


  • Amino acids levels (both essential and non essential)
  • Gastrointestinal markers
  • Detoxification markers
  • Neurological markers
  • Magnesium dependent markers
  • Urea cycle metabolites
  • B12, B6, folate dependent markers

Urine amino acid analysis in itself cannot be used as the ultimate diagnostic tool and it is normally supported by specific and further testing to confirm genetic and metabolic disorders. However, test reports will help in identifying the exact deficiencies that are contributing to disorders and allow for the precise replacement of those amino acids. The report will help nutritionists to draw a right supplement plan to replenish the required amino acids in the body.


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Collection of Pages - Last revised Date: September 21, 2020